1.0.0 P38634-1 1 1 1 FALSE SIC1 1 P38634 IID50275 FALSE 2 1 CDC28/CLB/CKS1 FALSE S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 FALSE 2 1 1 blocks cell cycle entry into S phase FALSE SIC1 1 P38634 IID50275 inhibition of S-CDK activity FALSE S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 FALSE 3 1 1 CDC28/CLN/CKS1 FALSE G1-CDK 1 P00546 2 P20437 3 P20438 4 P20486 FALSE 4 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 containing 10 CDK sites (T2, T5, T33, T45, T48, S69, S76, S80, T173, S191), phosphorylated stepwise by G1-CDK and S-CDK FALSE Phosphorylations 1 2 2 2 5 5 3 33 33 S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 FALSE 5 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 FALSE Phosphorylations 1 2 1 FALSE S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 S-CDK is released from Sic1-mediated inhibition, resulting in G1/S transition. FALSE 6 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 FALSE Phosphorylations 1 2 2 2 5 5 3 33 33 4 45 45 5 48 48 6 69 69 7 76 76 8 80 80 9 173 173 10 191 191 7 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 FALSE Phosphorylations 1 2 2 2 5 5 3 33 33 4 45 45 5 48 48 6 69 69 7 76 76 8 80 80 9 173 173 10 191 191 8 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 FALSE Phosphorylations 1 2 2 2 5 5 3 33 33 4 45 45 5 48 48 6 69 69 7 76 76 8 80 80 9 173 173 10 191 191 2 1 SCF(CDC4) complex + E2 FALSE SKP1 1 P52286 FALSE CDC4 1 P07834 FALSE CDC53 1 Q12018 FALSE CDC34 1 P14682 Ubiquitin-conjugating enzyme E2 FALSE 9 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 FALSE 3v7dE Phosphorylations 1 2 2 2 5 5 3 33 33 4 45 45 5 48 48 6 69 69 7 76 76 8 80 80 9 173 173 10 191 191 SKP1 1 P52286 FALSE 3v7dA CDC4 1 P07834 FALSE 3v7dB CDC53 1 Q12018 FALSE CDC34 1 P14682 FALSE 10 1 1 FALSE SKP1 1 P52286 FALSE CDC4 1 P07834 FALSE CDC53 1 Q12018 FALSE CDC34 1 P14682 FALSE 11 1 1 FALSE SIC1(Ps,Ub) 1 P38634 IID50275 Sic1 degradation FALSE Phosphorylations 1 2 2 2 5 5 3 33 33 4 45 45 5 48 48 6 69 69 7 76 76 8 80 80 9 173 173 10 191 191 Ubiquitination 1 SKP1 1 P52286 FALSE CDC4 1 P07834 FALSE CDC53 1 Q12018 FALSE CDC34 1 P14682 FALSE 12 1 1 FALSE S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 FALSE 13 1 1 FALSE S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 released S-CDK finalizes the phosphorylation cascade by completing the phosphorylation of the two di-phosphodegrons required for degradation (positive feedback). FALSE 14 1 1 FALSE SIC1(Ps) 1 P38634 IID50275 FALSE Phosphorylations 1 S-CDK 1 P00546 2 P30283 3 P32943 4 P20486 FALSE 1 1 FALSE 2 22356687 2 2 FALSE 3 4 32296067 3 4 TRUE 12 14 4 14 FALSE 5 5 7 FALSE 13 6 6 8 FALSE 9 22328159 19008353 7 9 TRUE 10 11 1 4 result 5 2 5 operator 13 1 2 4 result 5 1 5 operand 7 1 3 5 result 6 1 6 operand 8 1