Search Keyword:


Search option:


IID00023
 UniprotP12956
ProteinX-ray repair cross-complementing protein 6
GeneXRCC6
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
609
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-544 Hetero hexamer : IID00024Complex
 Evidence X-RAY 6erg D Reference
       Region 6erg D 1-31 disorder
       Region 6erg D 32-534 order
       Region 6erg D 535-544 disorder
 Evidence X-RAY 6erg A Reference
       Region 6erg A 1-33 disorder
       Region 6erg A 34-534 order
       Region 6erg A 535-544 disorder
Seq 1-544 Hetero hexamer : IID00024Complex
 Evidence X-RAY 6erh C Reference
       Region 6erh C 1-33 disorder
       Region 6erh C 34-52 order
       Region 6erh C 53-54 disorder
       Region 6erh C 55-158 order
       Region 6erh C 159-160 disorder
       Region 6erh C 161-225 order
       Region 6erh C 226-229 disorder
       Region 6erh C 230-534 order
       Region 6erh C 535-544 disorder
 Evidence X-RAY 6erh A Reference
       Region 6erh A 1-33 disorder
       Region 6erh A 34-51 order
       Region 6erh A 52-54 disorder
       Region 6erh A 55-156 order
       Region 6erh A 157-161 disorder
       Region 6erh A 162-226 order
       Region 6erh A 227-229 disorder
       Region 6erh A 230-534 order
       Region 6erh A 535-544 disorder
Seq 1-544 Hetero dodecamer : IID00024Complex
 Evidence X-RAY 6erf G Reference
       Region 6erf G 1-34 disorder
       Region 6erf G 35-156 order
       Region 6erf G 157-161 disorder
       Region 6erf G 162-534 order
       Region 6erf G 535-544 disorder
 Evidence X-RAY 6erf E Reference
       Region 6erf E 1-34 disorder
       Region 6erf E 35-158 order
       Region 6erf E 159-161 disorder
       Region 6erf E 162-310 order
       Region 6erf E 311-311 disorder
       Region 6erf E 312-535 order
       Region 6erf E 536-544 disorder
 Evidence X-RAY 6erf C Reference
       Region 6erf C 1-34 disorder
       Region 6erf C 35-157 order
       Region 6erf C 158-161 disorder
       Region 6erf C 162-538 order
       Region 6erf C 539-544 disorder
 Evidence X-RAY 6erf A Reference
       Region 6erf A 1-34 disorder
       Region 6erf A 35-156 order
       Region 6erf A 157-161 disorder
       Region 6erf A 162-308 order
       Region 6erf A 309-311 disorder
       Region 6erf A 312-535 order
       Region 6erf A 536-544 disorder
Seq 1-544 Hetero decamer : IID00024Complex,Q9BUH6
 Evidence X-RAY 8asc O Reference
       Region 8asc O 1-32 disorder
       Region 8asc O 33-223 order
       Region 8asc O 224-228 disorder
       Region 8asc O 229-535 order
       Region 8asc O 536-544 disorder
 Evidence X-RAY 8asc K Reference
       Region 8asc K 1-32 disorder
       Region 8asc K 33-231 order
       Region 8asc K 232-232 disorder
       Region 8asc K 233-535 order
       Region 8asc K 536-544 disorder
 Evidence X-RAY 8asc E Reference
       Region 8asc E 1-32 disorder
       Region 8asc E 33-223 order
       Region 8asc E 224-229 disorder
       Region 8asc E 230-535 order
       Region 8asc E 536-544 disorder
 Evidence X-RAY 8asc A Reference
       Region 8asc A 1-32 disorder
       Region 8asc A 33-227 order
       Region 8asc A 228-230 disorder
       Region 8asc A 231-534 order
       Region 8asc A 535-544 disorder
Seq 1-609 Hetero dimer : IID00024Complex
 Evidence X-RAY 1jeq A Reference
       Region 1jeq A 1-34 disorder
       Region 1jeq A 35-223 order
       Region 1jeq A 224-230 disorder
       Region 1jeq A 231-538 order
       Region 1jeq A 539-558 disorder
       Region 1jeq A 559-609 order
 Evidence X-RAY 1jey A Reference
       Region 1jey A 1-33 disorder
       Region 1jey A 34-222 order
       Region 1jey A 223-230 disorder
       Region 1jey A 231-534 order
       Region 1jey A 535-609 disorder
 Evidence X-RAY 7z6o A Reference
       Region 7z6o A 1-33 disorder
       Region 7z6o A 34-534 order
       Region 7z6o A 535-609 disorder
Seq 537-558 Hetero dimer : IID50009Complex
 Evidence X-RAY 3rzx B Reference
       Region 3rzx B 537-546 disorder
       Region 3rzx B 547-558 order
Seq 556-609 Monomer :
 Evidence NMR 1jjr A Reference
       Region 1jjr A 556-609 order
       Region 1jjr A 556-556 high_rmsd
SeqProS verified 547-558 Nuclear Localization Signal (NLS) Hetero dimer : IID50009Complex
       Region 3rzx B 547-558 order
       Region 1jeq A 539-558 disorder
Seqphosphorylation
    2-2 Phosphoserine
    6-6 Phosphoserine; by PRKDC
    27-27 Phosphoserine
    306-306 Phosphoserine
    51-51 Phosphoserine; by PRKDC
    560-560 Phosphoserine
    520-520 Phosphoserine
    550-550 Phosphoserine
    477-477 Phosphoserine
    455-455 Phosphothreonine
Seqacetylation
    2-2 N-acetylserine
    31-31 N6-acetyllysine
    338-338 N6-acetyllysine
    331-331 N6-acetyllysine
    461-461 N6-acetyllysine
 
Prediction
NeProc
Disorder 1-28,225-228,536-555
Order 29-224,229-535,556-609
ProS 1-13,225-228
AlphaFold
Disorder 1-34,54-54,78-78,225-231,536-560,592-592,609-609
Order 35-53,55-77,79-224,232-535,561-591,593-608
Pfam Hmmer
PF03731 37-256 2e-98
PF02735 261-468 3e-88
PF03730 471-559 1.9e-40
PF02037 573-607 2.2e-09
SEG 11-25 ,120-133 ,252-265 ,327-338
Function
Function in SwissProt
Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Required for double-strand break repair and V(D)J recombination (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Also has a role in chromosome translocation (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Has a role in chromosome translocation (PubMed:7957065, PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:8621488, PubMed:12145306, PubMed:11493912). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed:7957065, PubMed:8621488, PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:12145306, PubMed:11493912). It works in the 3'-5' direction (PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed:7957065, PubMed:8621488, PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:12145306, PubMed:11493912). Binding to DNA may be mediated by XRCC6 (PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912). The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed:20383123). 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed:20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:12145306). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).
Biological Process
Diagram with PDB data
XRCC6/XRCC5/NHEJ1Complex of XLF and heterodimer Ku bound to DNA