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IID00226
 UniprotQ9UKV5
ProteinE3 ubiquitin-protein ligase AMFR
GeneAMFR
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
643
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 313-393 Monomer :
 Evidence NMR 2lxh C Reference
       Region 2lxh C 313-326 disorder
       Region 2lxh C 327-384 order
       Region 2lxh C 384-384 high_rmsd
       Region 2lxh C 385-393 disorder
Seq 313-393,574-600 Hetero trimer : IID00307Complex
 Evidence X-RAY 4lad B Reference
       Region 4lad B 313-338 disorder
       Region 4lad B 339-380 order
       Region 4lad B 381-393 disorder
       Region 4lad B 574-599 order
       Region 4lad B 600-600 disorder
Seq 327-384,574-600 Hetero trimer : IID00307Complex
 Evidence NMR 2lxp B Reference
       Region 2lxp B 574-600 order
 Evidence NMR 2lxp C Reference
       Region 2lxp C 327-384 order
Seq 452-504 Monomer :
 Evidence NMR 2ejs A Reference
       Region 2ejs A 452-502 order
       Region 2ejs A 452-453 high_rmsd
       Region 2ejs A 502-502 high_rmsd
 Evidence NMR 2lvn C Reference
       Region 2lvn C 453-504 order
       Region 2lvn C 453-453 high_rmsd
       Region 2lvn C 502-504 high_rmsd
 Evidence X-RAY 4g3o A Reference
       Region 4g3o A 456-498 order
Seq 453-504 Hetero trimer : P0CG48
 Evidence NMR 2lvp C Reference
       Region 2lvp C 453-504 order
 Evidence NMR 2lvq D Reference
       Region 2lvq D 453-504 order
Seq 453-504 Hetero dimer : P0CG48
 Evidence NMR 2lvo C Reference
       Region 2lvo C 453-504 order
Seq 573-600 Hetero dimer : IID00307Complex
 Evidence X-RAY 3h8k B Reference
       Region 3h8k B 573-600 order
Seq 574-601 Hetero trimer : IID50078Complex
 Evidence X-RAY 3fsh C Reference
       Region 3fsh C 574-579 disorder
       Region 3fsh C 580-600 order
       Region 3fsh C 601-601 disorder
Seqdisorder 579-600
 Evidence NMR Reference
       Region 579-600 disorder
Seq 622-640 Hetero dimer : IID00201Complex
 Evidence X-RAY 3tiw C Reference
       Region 3tiw C 622-639 order
       Region 3tiw C 640-640 disorder
 Evidence X-RAY 3tiw D Reference
       Region 3tiw D 622-640 order
SeqProS verified 579-600 Hetero trimer : IID50078Complex
       Region 3fsh C 580-600 order
       Region 579-600 disorder
SeqProS verified 579-600 Hetero dimer : IID00307Complex
       Region 3h8k B 573-600 order
       Region 579-600 disorder
Seqphosphorylation
    542-542 Phosphoserine
    523-523 Phosphoserine
    516-516 Phosphoserine
 
Prediction
NeProc
Disorder 1-7,20-24,185-186,385-464,499-643
Order 8-19,25-42,49-184,187-384,465-498
ProS 1-7,20-24,185-186,418-438,567-571,580-600,623-639
AlphaFold
Disorder 1-12,39-67,285-287,337-338,384-424,429-430,433-434,436-436,438-453,499-571,573-573,601-621,640-643
Order 13-38,68-284,288-336,339-383,425-428,431-432,435-435,437-437,454-498,572-572,574-600,622-639
Pfam Hmmer
PF00097 341-378 2e-09
PF02845 456-497 5.9e-14
SEG 55-71 ,624-637
Function
Function in SwissProt
E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.