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IID00246
 UniprotQ14191
ProteinBifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
GeneWRN
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
1432
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 8-23 Hetero dimer : Q6DDS9
 Evidence X-RAY 6tyv B Reference
       Region 6tyv B 8-20 order
       Region 6tyv B 21-23 disorder
Seq 38-236 Monomer :
 Evidence X-RAY 2fbt A Reference
       Region 2fbt A 38-231 order
       Region 2fbt A 232-236 disorder
 Evidence X-RAY 2fbv A Reference
       Region 2fbv A 38-230 order
       Region 2fbv A 231-236 disorder
 Evidence X-RAY 2fbx A Reference
       Region 2fbx A 38-231 order
       Region 2fbx A 232-236 disorder
 Evidence X-RAY 2fby A Reference
       Region 2fby A 38-231 order
       Region 2fby A 232-236 disorder
 Evidence X-RAY 2fc0 A Reference
       Region 2fc0 A 38-231 order
       Region 2fc0 A 232-236 disorder
Seq 435-451 Hetero tetramer : IID00036Complex
 Evidence X-RAY 7xut B Reference
       Region 7xut B 435-451 order
 Evidence X-RAY 7xut A Reference
       Region 7xut A 435-449 order
       Region 7xut A 450-451 disorder
Seq 517-1093 Monomer :
 Evidence X-RAY 6yhr A Reference
       Region 6yhr A 517-527 disorder
       Region 6yhr A 528-949 order
       Region 6yhr A 950-953 disorder
       Region 6yhr A 954-1072 order
       Region 6yhr A 1073-1093 disorder
Seq 949-1079 Homo dimer :
 Evidence X-RAY 3aaf B Reference
       Region 3aaf B 949-955 disorder
       Region 3aaf B 956-1064 order
       Region 3aaf B 1065-1079 disorder
 Evidence X-RAY 3aaf A Reference
       Region 3aaf A 949-955 disorder
       Region 3aaf A 956-1064 order
       Region 3aaf A 1065-1079 disorder
Seq 949-1092 Monomer :
 Evidence NMR 2axl A Reference
       Region 2axl A 949-954 disorder
       Region 2axl A 949-953 high_rmsd
       Region 2axl A 955-1068 order
       Region 2axl A 1069-1092 disorder
       Region 2axl A 1073-1092 high_rmsd
Seq 1140-1242 Monomer :
 Evidence X-RAY 2e1e A Reference
       Region 2e1e A 1142-1235 order
       Region 2e1e A 1236-1242 disorder
 Evidence X-RAY 2e1f A Reference
       Region 2e1f A 1142-1235 order
       Region 2e1f A 1236-1242 disorder
 Evidence NMR 2dgz A Reference
       Region 2dgz A 1140-1239 order
       Region 2dgz A 1140-1141 high_rmsd
       Region 2dgz A 1235-1239 high_rmsd
SeqProS predicted 8-20 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5). Hetero dimer : Q6DDS9
       Region 6tyv B 8-20 order
SeqProS predicted 435-451 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5). Hetero tetramer : IID00036Complex
       Region 7xut A 435-449 order
       Region 7xut B 435-451 order
Seqphosphorylation
    426-426 Phosphoserine
    440-440 Phosphoserine
    453-453 Phosphoserine
    467-467 Phosphoserine
    478-478 Phosphoserine
    1133-1133 Phosphoserine
    1400-1400 Phosphoserine
Seqacetylation
    2-2 N-acetylserine
 
Prediction
NeProc
Disorder 1-30,305-529,1066-1085,1232-1251,1362-1432
Order 31-304,530-923,929-948,953-1065,1086-1137,1151-1231,1256-1361
ProS 13-30,309-318,325-379,384-419,427-505,525-529,1066-1074,1371-1376,1406-1414,1428-1432
AlphaFold
Disorder 1-42,88-94,231-251,253-254,257-258,261-262,264-282,292-528,621-625,677-678,800-800,823-827,919-919,921-927,948-956,1065-1065,1067-1067,1071-1145,1147-1147,1234-1254,1357-1432
Order 43-87,95-230,252-252,255-256,259-260,263-263,283-291,529-620,626-676,679-799,801-822,828-918,920-920,928-947,957-1064,1066-1066,1068-1070,1146-1146,1148-1233,1255-1356
Pfam Hmmer
PF01612 57-231 4.6e-55
PF00270 550-714 2.8e-28
PF00271 783-859 7.4e-26
PF00570 1150-1229 2.7e-27
SEG 399-414 ,506-526 ,1071-1080 ,1117-1129
Function
Function in SwissProt
Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed:9611231, PubMed:9224595, PubMed:9288107). Also has 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang (PubMed:11863428). Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA (PubMed:11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed:9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed:9288107, PubMed:9224595, PubMed:9611231). Depletion leads to chromosomal breaks and genome instability (PubMed:33199508).