Search Keyword:


Search option:


IID00848
 UniprotQ8IW19
ProteinAprataxin and PNK-like factor
GeneAPLF
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
511
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-105 Monomer :
 Evidence X-RAY 5w7w T Reference
       Region 5w7w T 1-104 order
       Region 5w7w T 105-105 disorder
 Evidence X-RAY 5w7w A Reference
       Region 5w7w A 1-103 order
       Region 5w7w A 104-105 disorder
Seq 1-105 Hetero dimer : IID00787Complex
 Evidence X-RAY 5w7x D Reference
       Region 5w7x D 1-2 disorder
       Region 5w7x D 3-104 order
       Region 5w7x D 105-105 disorder
 Evidence X-RAY 5w7x A Reference
       Region 5w7x A 1-2 disorder
       Region 5w7x A 3-103 order
       Region 5w7x A 104-105 disorder
 Evidence X-RAY 5w7x B Reference
       Region 5w7x B 1-2 disorder
       Region 5w7x B 3-104 order
       Region 5w7x B 105-105 disorder
 Evidence X-RAY 5w7x C Reference
       Region 5w7x C 1-2 disorder
       Region 5w7x C 3-104 order
       Region 5w7x C 105-105 disorder
 Evidence X-RAY 5w7y A Reference
       Region 5w7y A 1-2 disorder
       Region 5w7y A 3-105 order
 Evidence X-RAY 5w7y B Reference
       Region 5w7y B 1-2 disorder
       Region 5w7y B 3-105 order
Seq 1-105 Hetero tetramer : IID00828Complex
 Evidence X-RAY 5e50 B Reference
       Region 5e50 B 1-105 order
 Evidence X-RAY 5e50 A Reference
       Region 5e50 A 1-105 order
Seq 174-191 Hetero dodecamer : IID00024Complex
 Evidence X-RAY 6erf T Reference
       Region 6erf T 174-179 disorder
       Region 6erf T 180-191 order
 Evidence X-RAY 6erf S Reference
       Region 6erf S 174-179 disorder
       Region 6erf S 180-191 order
 Evidence X-RAY 6erf R Reference
       Region 6erf R 174-178 disorder
       Region 6erf R 179-191 order
 Evidence X-RAY 6erf Q Reference
       Region 6erf Q 174-179 disorder
       Region 6erf Q 180-191 order
Seq 179-194 Hetero dimer : Q6DDS9
 Evidence X-RAY 6tyw B Reference
       Region 6tyw B 179-180 disorder
       Region 6tyw B 181-193 order
       Region 6tyw B 194-194 disorder
 Evidence X-RAY 6tyz B Reference
       Region 6tyz B 179-180 disorder
       Region 6tyz B 181-192 order
       Region 6tyz B 193-194 disorder
Seq 179-194 Hetero trimer : Q6DDS9
 Evidence X-RAY 6tyt C Reference
       Region 6tyt C 179-181 disorder
       Region 6tyt C 182-192 order
       Region 6tyt C 193-194 disorder
Seq 360-448 Monomer :
 Evidence NMR 2kuo A Reference
       Region 2kuo A 360-448 order
       Region 2kuo A 360-373 high_rmsd
       Region 2kuo A 415-448 high_rmsd
Seq 368-451 Monomer :
 Evidence NMR 2kqc A Reference
       Region 2kqc A 368-404 disorder
       Region 2kqc A 405-451 order
       Region 2kqc A 405-413 high_rmsd
       Region 2kqc A 444-451 high_rmsd
 Evidence NMR 2kqe A Reference
       Region 2kqe A 368-404 disorder
       Region 2kqe A 405-451 order
       Region 2kqe A 405-413 high_rmsd
       Region 2kqe A 445-451 high_rmsd
Seq 368-451 Monomer :
 Evidence NMR 2kqb A Reference
       Region 2kqb A 368-417 order
       Region 2kqb A 368-369 high_rmsd
       Region 2kqb A 408-417 high_rmsd
       Region 2kqb A 418-451 disorder
 Evidence NMR 2kqd A Reference
       Region 2kqd A 368-417 order
       Region 2kqd A 368-369 high_rmsd
       Region 2kqd A 408-417 high_rmsd
       Region 2kqd A 418-451 disorder
Seq 449-490 Hetero 40mer : IID50135Complex,IID50145Complex,P02302,Q6AZJ8
 Evidence X-RAY 6yn1 n Reference
       Region 6yn1 n 449-457 disorder
       Region 6yn1 n 458-471 order
       Region 6yn1 n 472-481 disorder
       Region 6yn1 n 482-487 order
       Region 6yn1 n 488-490 disorder
 Evidence X-RAY 6yn1 i Reference
       Region 6yn1 i 449-458 disorder
       Region 6yn1 i 459-473 order
       Region 6yn1 i 474-481 disorder
       Region 6yn1 i 482-487 order
       Region 6yn1 i 488-490 disorder
 Evidence X-RAY 6yn1 d Reference
       Region 6yn1 d 449-458 disorder
       Region 6yn1 d 459-472 order
       Region 6yn1 d 473-481 disorder
       Region 6yn1 d 482-487 order
       Region 6yn1 d 488-490 disorder
 Evidence X-RAY 6yn1 Y Reference
       Region 6yn1 Y 449-458 disorder
       Region 6yn1 Y 459-474 order
       Region 6yn1 Y 475-481 disorder
       Region 6yn1 Y 482-487 order
       Region 6yn1 Y 488-490 disorder
 Evidence X-RAY 6yn1 T Reference
       Region 6yn1 T 449-458 disorder
       Region 6yn1 T 459-473 order
       Region 6yn1 T 474-480 disorder
       Region 6yn1 T 481-487 order
       Region 6yn1 T 488-490 disorder
 Evidence X-RAY 6yn1 O Reference
       Region 6yn1 O 449-458 disorder
       Region 6yn1 O 459-473 order
       Region 6yn1 O 474-481 disorder
       Region 6yn1 O 482-487 order
       Region 6yn1 O 488-490 disorder
 Evidence X-RAY 6yn1 J Reference
       Region 6yn1 J 449-458 disorder
       Region 6yn1 J 459-487 order
       Region 6yn1 J 488-490 disorder
 Evidence X-RAY 6yn1 E Reference
       Region 6yn1 E 449-458 disorder
       Region 6yn1 E 459-474 order
       Region 6yn1 E 475-481 disorder
       Region 6yn1 E 482-487 order
       Region 6yn1 E 488-490 disorder
Seqdisorder 450-495
 Evidence NMR Reference
       Region 450-495 disorder
SeqProS verified 458-487 Hetero : IID50135Complex,IID50145Complex,P02302,Q6AZJ8
       Region 6yn1 E 459-474 order
       Region 6yn1 E 482-487 order
       Region 6yn1 J 459-487 order
       Region 6yn1 O 459-473 order
       Region 6yn1 O 482-487 order
       Region 6yn1 T 459-473 order
       Region 6yn1 T 481-487 order
       Region 6yn1 Y 459-474 order
       Region 6yn1 Y 482-487 order
       Region 6yn1 d 459-472 order
       Region 6yn1 d 482-487 order
       Region 6yn1 i 459-473 order
       Region 6yn1 i 482-487 order
       Region 6yn1 n 458-471 order
       Region 6yn1 n 482-487 order
       Region 450-495 disorder
Seqphosphorylation
    116-116 Phosphoserine; by ATM
    149-149 Phosphoserine
 
Prediction
NeProc
Disorder 1-1,110-511
Order 2-66,71-109
ProS 110-119,135-140,168-173,182-195,224-229,250-278,288-299,312-327,380-408,414-438,445-467,482-488,495-511
AlphaFold
Disorder 1-2,105-183,193-374,406-416,442-483,486-495,511-511
Order 3-104,184-192,375-405,417-441,484-485,496-510
SEG 345-355 ,470-484 ,489-503
Function
Function in SwissProt
Histone chaperone involved in single-strand and double-strand DNA break repair (PubMed:17353262, PubMed:17396150, PubMed:21211721, PubMed:21211722, PubMed:30104678, PubMed:29905837). Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions (PubMed:17353262, PubMed:17396150, PubMed:21211721, PubMed:30104678). Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1 (PubMed:21211722, PubMed:30104678, PubMed:29905837). Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro (PubMed:17353262, PubMed:17396150). Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage (PubMed:17353262, PubMed:17396150). Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed:21211721, PubMed:23689425). Also acts as a negative regulator of cell pluripotency by promoting histone exchange (By similarity). Required for the embryo implantation during the epithelial to mesenchymal transition in females (By similarity).
Biological Process
See also
Diagram with PDB data
XRCC6/XRCC5/NHEJ1Complex of XLF and heterodimer Ku bound to DNA