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IID50368
 UniprotP40343
ProteinVacuolar protein sorting-associated protein 27
GeneVPS27
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
622
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 163-230 Monomer :
 Evidence X-RAY 1vfy A Reference
       Region 1vfy A 163-168 disorder
       Region 1vfy A 169-230 order
Seq 249-329 Monomer :
 Evidence NMR 1q0v A Reference
       Region 1q0v A 249-329 order
Seq 255-278 Hetero dimer : P0CG63
 Evidence NMR 1q0w A Reference
       Region 1q0w A 255-278 order
Seq 256-278 Hetero dimer : J3QS39
 Evidence X-RAY 6njg B Reference
       Region 6njg B 256-271 order
       Region 6njg B 272-278 disorder
Seq 258-277 Hetero dimer : P0CG63
 Evidence NMR 2kdi A Reference
       Region 2kdi A 258-277 order
       Region 2kdi A 277-277 high_rmsd
Seq 301-320 Monomer :
 Evidence X-RAY 1o06 A Reference
       Region 1o06 A 301-320 order
Seq 348-438 Hetero dimer : IID50366Complex
 Evidence X-RAY 2pjw V Reference
       Region 2pjw V 348-438 order
Seq 445-453 Hetero dimer : IID50362Complex
 Evidence X-RAY 3r42 B Reference
       Region 3r42 B 445-452 order
       Region 3r42 B 453-453 disorder
SeqProS predicted 445-452 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5). Hetero dimer : IID50362Complex
       Region 3r42 B 445-452 order
Seqphosphorylation
    157-157 Phosphoserine
    495-495 Phosphoserine
    613-613 Phosphoserine
 
Prediction
NeProc
Disorder 1-5,156-207,213-306,335-376,433-622
Order 6-151,208-212,307-334,377-432
ProS 166-185,195-202,213-230,260-269,304-306,345-349,356-376,579-582,616-622
AlphaFold
Disorder 1-1,104-106,130-130,150-168,235-259,274-303,306-306,310-310,328-348,372-378,436-612,615-615,617-619,621-622
Order 2-103,107-129,131-149,169-234,260-273,304-305,307-309,311-327,349-371,379-435,613-614,616-616,620-620
Pfam Hmmer
PF00790 3-145 7.2e-61
PF01363 168-231 7e-34
SEG 232-252 ,334-344
Function
Function in SwissProt
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane (PubMed:3062374, PubMed:1493335, PubMed:9265642, PubMed:11416128, PubMed:12055639, PubMed:11872141, PubMed:12900393, PubMed:14581452, PubMed:15166140, PubMed:15107463, PubMed:15086794, PubMed:17135292, PubMed:18508771). Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi (PubMed:11208109, PubMed:8649377, PubMed:9015300). Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC (PubMed:8649377). Might also function as an alternate adapter in the COPIb clathrin-like coat (PubMed:17101773).
Biological Process
Diagram with PDB data
VPS27/HSE1The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting
See also
Diagram with PDB data
STP22/VPS27Crystal structure of the yeast vps23 UEV domain in complex with a vps27 PSDP peptide
VPS36/SNF8/VPS25/VPS25The crystal structure of endosomal complex ESCRT-II (VPS22/VPS25/VPS36)
Diagram without PDB data
VPS20ESCRT-III recruitment