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IID50385
UniprotQ04272
ProteinVacuolar protein sorting-associated protein 20
GeneVPS20
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
221
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 170-195 Hetero dimer : IID50372Complex
 Evidence X-RAY 5fvl D Reference
       Region 5fvl D 170-195 order
 Evidence X-RAY 5fvl C Reference
       Region 5fvl C 170-192 order
       Region 5fvl C 193-195 disorder
SeqProS predicted 170-195 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5). Hetero dimer : IID50372Complex
       Region 5fvl C 170-192 order
       Region 5fvl D 170-195 order
 
Prediction
NeProc
Disorder 1-11,62-67,141-221
Order 12-61,68-140
ProS 62-67,141-148,155-169,187-193,215-221
AlphaFold
Disorder 1-7,120-127,129-129,133-133,137-137,140-141,144-156,171-221
Order 8-119,128-128,130-132,134-136,138-139,142-143,157-170
Pfam Hmmer
PF03357 15-196 5.3e-58
SEG 155-169
Function
Function in SwissProt
Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Required for the oligomerization of SNF7 into a membrane-associated filament. The VPS20-SNF7 subcomplex is responsible for the membrane association of the ESCRT-III complex. Also required for the RIM101 repressor proteolytic activation.
Biological Process
Diagram without PDB data
VPS20ESCRT-III recruitment
See also
Diagram with PDB data
VPS4/VTA1/DID4Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p