MOLECULAR BASIS FOR SYNERGISTIC TRANSCRIPTIONAL ACTIVATION BY OCT1 AND SOX2 REVEALED FROM THE SOLUTION STRUCTURE OF THE 42-KDA OCT1.SOX2.HOXB1-DNA TERNARY TRANSCRIPTION FACTOR COMPLEX.

20210801 20180803 20180328 20210801 IID00626 Transcription factor SOX-2 Homo sapiens 317 P48431 Q14537 Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity). Nucleus. SL-0191 unkown 0 0 Phosphoserine 251 251 SOX2 SOX2_HUMAN MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM 1 1o4xB 39 115 order 1 2 1o4xB 116 121 disorder 1 4 2le4A 39 104 order 2 5 2le4A 105 118 disorder 2 6 2le4A 39 42 high_rmsd 2 7 2le4A 105 118 high_rmsd 2 1 1o4x NMR 14559893 D.C.WILLIAMS,M.CAI,G.M.CLORE MOLECULAR BASIS FOR SYNERGISTIC TRANSCRIPTIONAL ACTIVATION BY OCT1 AND SOX2 REVEALED FROM THE SOLUTION STRUCTURE OF THE 42-KDA OCT1.SOX2.HOXB1-DNA TERNARY TRANSCRIPTION FACTOR COMPLEX. 2004 J.BIOL.CHEM. 279 1449 nmr 1o4x B 39 121 100 1 116 121 1 1o4x 3815 Hetero dimer 1o4xA P14859 IID00144 1o4xB P48431 IID00626 1o4xA-1o4xB 2 2le4 NMR S.SAHU,J.MARKLEY,M.TONELLI,A.BAHRAMI,H.EGHBALNIA SOLUTION STRUCTURE OF THE HMG BOX DNA-BINDING DOMAIN OF HUMAN STEM CELL TRANSCRIPTION FACTOR SOX2 TO BE PUBLISHED nmr 2le4 A 39 118 100 0 1 2le4_1 2548 Monomer monomer 2le4A P48431 IID00626 ProS 1 verified 1 105 115 5 105 118 This region is ordered when it binds upon DNA.

P48431-1 SOX2/POU2F1/DNA TERNARY COMPLEX OF THE DNA BINDING DOMAINS OF THE OCT1 AND SOX2 TRANSCRIPTION FACTORS WITH A 19MER OLIGONUCLEOTIDE FROM THE HOXB1 REGULATORY ELEMENT P48431-2 SOX2/KPNA4 SOX2 bound to Importin-alpha 3

39-118,272-274 1-38,119-271,275-317 45-107 1-44,108-317 1-10,37-44,108-112,153-166,198-201,267-280,310-317 12-33,135-154,246-261