20250201 IID50385 Vacuolar protein sorting-associated protein 20 Amino acid sensor-independent protein 10 Charged multivesicular body protein 6 ESCRT-III complex subunit VPS20 Vacuolar protein-targeting protein 20 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 221 Q04272 D6VZQ1 Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Required for the oligomerization of SNF7 into a membrane-associated filament. The VPS20-SNF7 subcomplex is responsible for the membrane association of the ESCRT-III complex. Also required for the RIM101 repressor proteolytic activation. 0 0 0 0 VPS20 Vacuolar protein sorting-associa MGQKSSKVHITKTDRAILEVKRSKDEIHKFTRRTDNLILVEKSQLKDLIRKNPENYKSNMKVRFLLKRIHYQEHLLQQASDQLINLENMVSTLEFKMVEKQFINGLKNGNEILKKLNKEFSNVDELMDDVQDQIAYQNEINETLSRSLVGTSNYEDDLDKELDALESELNPEKMNNAKVANMPSTEGLPSLPQGEQTEQKEREEFATEERSDTKEPLALLS 1 5fvlC 170 192 order 1 2 5fvlC 193 195 disorder 1 3 5fvlD 170 195 order 1 1 5fvl X-RAY 8.6 27075672 R.KOJIMA,T.OBITA,K.ONOUE,M.MIZUGUCHI STRUCTURAL FINE-TUNING OF MIT INTERACTING MOTIF 2 (MIM2) AND ALLOSTERIC REGULATION OF ESCRT-III BY VPS4 IN YEAST. 2016 J.MOL.BIOL. 428 2392 x-ray 5fvl C 170 195 100 1 193 195 x-ray 5fvl D 170 195 100 0 1 5fvl_1 1575 Hetero dimer 5fvlA P52917 IID50372 5fvlD Q04272 IID50385 5fvlD-5fvlA 2 5fvl_2 1575 Hetero dimer 5fvlB P52917 IID50372 5fvlC Q04272 IID50385 5fvlC-5fvlB ProS 1 predicted 1,3 170 195 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5).
Q04272-1 VPS20 ESCRT-III recruitment
P52917-1 VPS4/VTA1/DID4 Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 8-119,128-128,130-132,134-136,138-139,142-143,157-170 1-7,120-127,129-129,133-133,137-137,140-141,144-156,171-221 12-61,68-140 1-11,62-67,141-221 62-67,141-148,155-169,187-193,215-221 155-169