Search Keyword:


Search option:


IID01016
 UniprotQ9GZZ9
ProteinUbiquitin-like modifier-activating enzyme 5
GeneUBA5
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
404
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 36-335 Homo hexadecamer :
 Evidence X-RAY 6h78 A Reference
       Region 6h78 A 36-243 order
       Region 6h78 A 244-247 disorder
       Region 6h78 A 248-322 order
       Region 6h78 A 323-335 disorder
 Evidence X-RAY 6h78 B Reference
       Region 6h78 B 36-238 order
       Region 6h78 B 239-246 disorder
       Region 6h78 B 247-321 order
       Region 6h78 B 322-335 disorder
 Evidence X-RAY 6h78 C Reference
       Region 6h78 C 36-321 order
       Region 6h78 C 322-335 disorder
 Evidence X-RAY 6h78 D Reference
       Region 6h78 D 36-232 order
       Region 6h78 D 233-246 disorder
       Region 6h78 D 247-321 order
       Region 6h78 D 322-335 disorder
 Evidence X-RAY 6h78 E Reference
       Region 6h78 E 36-321 order
       Region 6h78 E 322-335 disorder
 Evidence X-RAY 6h78 F Reference
       Region 6h78 F 36-232 order
       Region 6h78 F 233-247 disorder
       Region 6h78 F 248-321 order
       Region 6h78 F 322-335 disorder
 Evidence X-RAY 6h78 G Reference
       Region 6h78 G 36-238 order
       Region 6h78 G 239-248 disorder
       Region 6h78 G 249-321 order
       Region 6h78 G 322-335 disorder
 Evidence X-RAY 6h78 H Reference
       Region 6h78 H 36-243 order
       Region 6h78 H 244-247 disorder
       Region 6h78 H 248-319 order
       Region 6h78 H 320-335 disorder
 Evidence X-RAY 6h78 I Reference
       Region 6h78 I 36-244 order
       Region 6h78 I 245-249 disorder
       Region 6h78 I 250-320 order
       Region 6h78 I 321-335 disorder
 Evidence X-RAY 6h78 J Reference
       Region 6h78 J 36-238 order
       Region 6h78 J 239-245 disorder
       Region 6h78 J 246-321 order
       Region 6h78 J 322-335 disorder
 Evidence X-RAY 6h78 K Reference
       Region 6h78 K 36-236 order
       Region 6h78 K 237-246 disorder
       Region 6h78 K 247-321 order
       Region 6h78 K 322-335 disorder
 Evidence X-RAY 6h78 L Reference
       Region 6h78 L 36-243 order
       Region 6h78 L 244-248 disorder
       Region 6h78 L 249-320 order
       Region 6h78 L 321-335 disorder
 Evidence X-RAY 6h78 M Reference
       Region 6h78 M 36-232 order
       Region 6h78 M 233-248 disorder
       Region 6h78 M 249-321 order
       Region 6h78 M 322-335 disorder
 Evidence X-RAY 6h78 N Reference
       Region 6h78 N 36-321 order
       Region 6h78 N 322-335 disorder
 Evidence X-RAY 6h78 O Reference
       Region 6h78 O 36-232 order
       Region 6h78 O 233-247 disorder
       Region 6h78 O 248-321 order
       Region 6h78 O 322-335 disorder
 Evidence X-RAY 6h78 P Reference
       Region 6h78 P 36-321 order
       Region 6h78 P 322-335 disorder
Seq 36-346 Hetero octamer : P61960
 Evidence X-RAY 6h77 A Reference
       Region 6h77 A 36-321 order
       Region 6h77 A 322-331 disorder
       Region 6h77 A 332-346 order
 Evidence X-RAY 6h77 B Reference
       Region 6h77 B 36-323 order
       Region 6h77 B 324-332 disorder
       Region 6h77 B 333-346 order
 Evidence X-RAY 6h77 C Reference
       Region 6h77 C 36-322 order
       Region 6h77 C 323-332 disorder
       Region 6h77 C 333-346 order
 Evidence X-RAY 6h77 D Reference
       Region 6h77 D 36-322 order
       Region 6h77 D 323-332 disorder
       Region 6h77 D 333-346 order
Seq 57-329 Homo dimer :
 Evidence X-RAY 3guc A Reference
       Region 3guc A 57-68 disorder
       Region 3guc A 69-109 order
       Region 3guc A 110-117 disorder
       Region 3guc A 118-236 order
       Region 3guc A 237-245 disorder
       Region 3guc A 246-322 order
       Region 3guc A 323-324 disorder
       Region 3guc A 325-329 order
 Evidence X-RAY 3guc B Reference
       Region 3guc B 57-68 disorder
       Region 3guc B 69-106 order
       Region 3guc B 107-124 disorder
       Region 3guc B 125-238 order
       Region 3guc B 239-250 disorder
       Region 3guc B 251-319 order
       Region 3guc B 320-329 disorder
 Evidence X-RAY 3h8v A Reference
       Region 3h8v A 57-68 disorder
       Region 3h8v A 69-108 order
       Region 3h8v A 109-120 disorder
       Region 3h8v A 121-238 order
       Region 3h8v A 239-245 disorder
       Region 3h8v A 246-318 order
       Region 3h8v A 319-329 disorder
 Evidence X-RAY 3h8v B Reference
       Region 3h8v B 57-69 disorder
       Region 3h8v B 70-106 order
       Region 3h8v B 107-124 disorder
       Region 3h8v B 125-237 order
       Region 3h8v B 238-248 disorder
       Region 3h8v B 249-320 order
       Region 3h8v B 321-329 disorder
Seq 57-346 Hetero tetramer : P61960
 Evidence X-RAY 5iaa A Reference
       Region 5iaa A 57-67 disorder
       Region 5iaa A 68-112 order
       Region 5iaa A 113-115 disorder
       Region 5iaa A 116-243 order
       Region 5iaa A 244-249 disorder
       Region 5iaa A 250-322 order
       Region 5iaa A 323-332 disorder
       Region 5iaa A 333-346 order
 Evidence X-RAY 5iaa B Reference
       Region 5iaa B 57-67 disorder
       Region 5iaa B 68-106 order
       Region 5iaa B 107-123 disorder
       Region 5iaa B 124-242 order
       Region 5iaa B 243-252 disorder
       Region 5iaa B 253-322 order
       Region 5iaa B 323-335 disorder
       Region 5iaa B 336-346 order
 Evidence X-RAY 5l95 A Reference
       Region 5l95 A 68-110 order
       Region 5l95 A 111-116 disorder
       Region 5l95 A 117-243 order
       Region 5l95 A 244-252 disorder
       Region 5l95 A 253-323 order
       Region 5l95 A 324-332 disorder
       Region 5l95 A 333-346 order
 Evidence X-RAY 5l95 B Reference
       Region 5l95 B 68-106 order
       Region 5l95 B 107-123 disorder
       Region 5l95 B 124-242 order
       Region 5l95 B 243-252 disorder
       Region 5l95 B 253-322 order
       Region 5l95 B 323-335 disorder
       Region 5l95 B 336-346 order
Seq 333-348 Hetero dimer : IID00955Complex
 Evidence NMR 6h8c B Reference
       Region 6h8c B 333-348 order
       Region 6h8c B 333-333 high_rmsd
Seq 334-346 Hetero trimer : P61960
 Evidence X-RAY 5ia8 B The regions 349-374 of 5ia8A and B are derived from the chimera partner: P61960, whereas misassigned to Q9GZZ9 in UniProt. Reference
       Region 5ia8 B 334-334 disorder
       Region 5ia8 B 335-346 order
Seq 334-346 Hetero trimer : P61960
 Evidence X-RAY 5ia8 A The regions 349-374 of 5ia8A and B are derived from the chimera partner: P61960, whereas misassigned to Q9GZZ9 in UniProt. Reference
       Region 5ia8 A 334-346 order
Seq 338-346 Hetero trimer : P61960
 Evidence X-RAY 5hkh B Reference
       Region 5hkh B 338-346 order
Seq 381-404 Hetero dimer : Q9Y3C8
 Evidence NMR 7ovc B Reference
       Region 7ovc B 381-404 order
SeqProS predicted 381-391 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5). Hetero dimer : Q9Y3C8
       Region 7ovc B 381-404 order
Seqphosphorylation
    393-393 Phosphoserine
    358-358 Phosphoserine
    45-45 Phosphoserine
 
Prediction
NeProc
Disorder 1-49,320-404
Order 50-319
ProS 1-25,31-49,320-326,332-359,364-375,391-404
AlphaFold
Disorder 1-2,24-35,324-332,348-391,404-404
Order 3-23,36-323,333-347,392-403
SEG 79-87 ,326-340 ,344-354
Function
Function in SwissProt
E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:25219498, PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:27545674, PubMed:30412706, PubMed:27545681). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677). Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:32160526). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).