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IID01023
 UniprotQ9NT62
ProteinUbiquitin-like-conjugating enzyme ATG3
GeneATG3
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
314
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 25-89,191-314 Monomer :
 Evidence NMR 8fkm A Reference
       Region 8fkm A 25-89 order
       Region 8fkm A 191-314 order
       Region 8fkm A 210-213 high_rmsd
Seq 90-113 Hetero hexadecamer : IID00921Complex
 Evidence X-RAY 8afi B Reference
       Region 8afi B 90-94 disorder
       Region 8afi B 95-110 order
       Region 8afi B 111-113 disorder
 Evidence X-RAY 8afi D Reference
       Region 8afi D 90-92 disorder
       Region 8afi D 93-110 order
       Region 8afi D 111-113 disorder
 Evidence X-RAY 8afi F Reference
       Region 8afi F 90-95 disorder
       Region 8afi F 96-111 order
       Region 8afi F 112-113 disorder
 Evidence X-RAY 8afi J Reference
       Region 8afi J 90-94 disorder
       Region 8afi J 95-111 order
       Region 8afi J 112-113 disorder
 Evidence X-RAY 8afi L Reference
       Region 8afi L 90-95 disorder
       Region 8afi L 96-110 order
       Region 8afi L 111-113 disorder
 Evidence X-RAY 8afi H Reference
       Region 8afi H 90-103 disorder
       Region 8afi H 104-111 order
       Region 8afi H 112-113 disorder
 Evidence X-RAY 8afi N Reference
       Region 8afi N 90-102 disorder
       Region 8afi N 103-111 order
       Region 8afi N 112-113 disorder
 Evidence X-RAY 8afi P Reference
       Region 8afi P 90-103 disorder
       Region 8afi P 104-110 order
       Region 8afi P 111-113 disorder
Seqdisorder 94-112
 Evidence CD Reference
       Region 94-112 disorder
Seq 140-170 Hetero tetramer : O94817
 Evidence X-RAY 4naw D Reference
       Region 4naw D 140-152 disorder
       Region 4naw D 153-165 order
       Region 4naw D 166-170 disorder
 Evidence X-RAY 4naw H Reference
       Region 4naw H 140-153 disorder
       Region 4naw H 154-165 order
       Region 4naw H 166-170 disorder
 Evidence X-RAY 4naw L Reference
       Region 4naw L 140-153 disorder
       Region 4naw L 154-165 order
       Region 4naw L 166-170 disorder
 Evidence X-RAY 4naw P Reference
       Region 4naw P 140-152 disorder
       Region 4naw P 153-165 order
       Region 4naw P 166-170 disorder
SeqProS verified 93-111 Hetero hexadecamer : IID00921Complex
       Region 8afi B 95-110 order
       Region 8afi D 93-110 order
       Region 8afi F 96-111 order
       Region 8afi H 104-111 order
       Region 8afi J 95-111 order
       Region 8afi L 96-110 order
       Region 8afi N 103-111 order
       Region 8afi P 104-110 order
       Region 94-112 disorder
Seqacetylation
    1-1 N-acetylmethionine
 
Prediction
NeProc
Disorder 117-135,164-184
Order 1-116,136-163,185-314
ProS 117-134,164-184
AlphaFold
Disorder 1-14,87-155,163-194,309-314
Order 15-86,156-162,195-308
SEG 144-162
Function
Function in SwissProt
E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation (PubMed:24191030, PubMed:37252361, PubMed:33446636). Cycles back and forth between binding to ATG7 for loading with the ATG8-like proteins and binding to E3 enzyme, composed of ATG12, ATG5 and ATG16L1 to promote ATG8-like proteins lipidation (PubMed:12207896, PubMed:24186333, PubMed:11825910, PubMed:12890687, PubMed:16704426). Also plays a role as a membrane curvature sensor that facilitates LC3/GABARAP lipidation by sensing local membrane stress associated with lipid-packing defects as occurs with high molar proportions of conical lipids or strident membrane curvature (By similarity). Interacts with negatively-charged membranes promoting membrane tethering and enhancing LC3/GABARAP lipidation (PubMed:29142222). Also acts as an autocatalytic E2-like enzyme by catalyzing the conjugation of ATG12 to itself in an ATG7-dependent manner, this complex thus formed, plays a role in mitochondrial homeostasis but not in autophagy (By similarity). ATG12-ATG3 conjugation promotes late endosome to lysosome trafficking and basal autophagosome maturation via its interaction with PDCD6IP (By similarity). ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation (By similarity). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (By similarity).