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IID01028
 UniprotQ9UHB7
ProteinAF4/FMR2 family member 4
GeneAFF4
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
1163
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 2-73 Hetero tetramer : IID90039Complex,O60563
 Evidence X-RAY 4ogr G Reference
       Region 4ogr G 2-33 disorder
       Region 4ogr G 34-69 order
       Region 4ogr G 70-73 disorder
Seq 2-73 Hetero tetramer : IID90039Complex,O60563,P50750
 Evidence X-RAY 4ogr C Reference
       Region 4ogr C 2-3 disorder
       Region 4ogr C 4-21 order
       Region 4ogr C 22-32 disorder
       Region 4ogr C 33-69 order
       Region 4ogr C 70-73 disorder
 Evidence X-RAY 4ogr L Reference
       Region 4ogr L 2-3 disorder
       Region 4ogr L 4-21 order
       Region 4ogr L 22-33 disorder
       Region 4ogr L 34-69 order
       Region 4ogr L 70-73 disorder
Seq 2-73 Hetero trimer : O60563,P50750
 Evidence X-RAY 4imy H Reference
       Region 4imy H 2-2 disorder
       Region 4imy H 3-21 order
       Region 4imy H 22-33 disorder
       Region 4imy H 34-67 order
       Region 4imy H 68-73 disorder
Seq 2-73 Hetero trimer : O60563
 Evidence X-RAY 4imy G Reference
       Region 4imy G 2-33 disorder
       Region 4imy G 34-66 order
       Region 4imy G 67-73 disorder
 Evidence X-RAY 4imy I Reference
       Region 4imy I 2-33 disorder
       Region 4imy I 34-66 order
       Region 4imy I 67-73 disorder
Seq 32-67 Hetero tetramer : IID90038Complex,O60563
 Evidence X-RAY 5l1z C Reference
       Region 5l1z C 32-33 disorder
       Region 5l1z C 34-67 order
Seq 32-69 Hetero tetramer : IID90033Complex,O60563
 Evidence X-RAY 4or5 E Reference
       Region 4or5 E 32-69 order
 Evidence X-RAY 4or5 J Reference
       Region 4or5 J 32-69 order
 Evidence X-RAY 6cyt C Reference
       Region 6cyt C 32-33 disorder
       Region 6cyt C 34-64 order
       Region 6cyt C 65-67 disorder
Seq 301-351 Hetero dimer : O00472
 Evidence X-RAY 5jw9 A Reference
       Region 5jw9 A 301-313 disorder
       Region 5jw9 A 314-349 order
       Region 5jw9 A 350-351 disorder
Seqdisorder 2-73,318-337,710-729
 Evidence CD Reference
       Region 2-73 disorder
       Region 318-337 disorder
       Region 710-729 disorder
Seq 899-1163 Monomer :
 Evidence X-RAY 6k7p A Reference
       Region 6k7p A 902-902 disorder
       Region 6k7p A 903-960 order
       Region 6k7p A 961-964 disorder
       Region 6k7p A 965-1045 order
       Region 6k7p A 1046-1081 disorder
       Region 6k7p A 1082-1158 order
       Region 6k7p A 1159-1163 disorder
 Evidence X-RAY 6kn5 A Reference
       Region 6kn5 A 899-901 disorder
       Region 6kn5 A 902-1046 order
       Region 6kn5 A 1047-1080 disorder
       Region 6kn5 A 1081-1160 order
       Region 6kn5 A 1161-1163 disorder
 Evidence X-RAY 6r80 A Reference
       Region 6r80 A 899-900 disorder
       Region 6r80 A 901-960 order
       Region 6r80 A 961-964 disorder
       Region 6r80 A 965-984 order
       Region 6r80 A 985-985 disorder
       Region 6r80 A 986-1047 order
       Region 6r80 A 1048-1080 disorder
       Region 6r80 A 1081-1162 order
       Region 6r80 A 1163-1163 disorder
SeqProS verified 3-69 Hetero tetramer : IID90039Complex,O60563,P50750
       Region 4ogr C 4-21 order
       Region 4ogr C 33-69 order
       Region 4ogr L 4-21 order
       Region 4ogr L 34-69 order
       Region 2-73 disorder
SeqProS verified 3-69 Hetero trimer : O60563,P50750
       Region 4imy H 3-21 order
       Region 4imy H 34-67 order
       Region 2-73 disorder
SeqProS verified 3-69 Hetero trimer : O60563
       Region 4imy G 34-66 order
       Region 4imy I 34-66 order
       Region 2-73 disorder
SeqProS verified 3-69 Hetero tetramer : IID90039Complex,O60563
       Region 4ogr G 34-69 order
       Region 2-73 disorder
SeqProS verified 32-69 Hetero tetramer : IID90033Complex,O60563
       Region 4or5 E 32-69 order
       Region 6cyt C 34-64 order
       Region 2-73 disorder
SeqProS verified 34-67 Hetero tetramer : IID90038Complex,O60563
       Region 5l1z C 34-67 order
       Region 2-73 disorder
SeqProS verified 318-337 Hetero dimer : O00472
       Region 5jw9 A 314-349 order
       Region 318-337 disorder
SeqProS predicted 338-349 This region is predicted to be disordered by NeProc and AlphaFold (pLDDT < 68.5). Hetero dimer : O00472
       Region 5jw9 A 314-349 order
Seqphosphorylation
    392-392 Phosphoserine
    674-674 Phosphothreonine
    671-671 Phosphoserine
    549-549 Phosphoserine
    491-491 Phosphoserine
    490-490 Phosphoserine
    487-487 Phosphoserine
    389-389 Phosphoserine
    388-388 Phosphoserine
    387-387 Phosphoserine
    212-212 Phosphoserine
    120-120 Phosphoserine
    703-703 Phosphoserine
    814-814 Phosphoserine
    836-836 Phosphoserine
    1043-1043 Phosphoserine
    1055-1055 Phosphoserine
    1058-1058 Phosphoserine
    1062-1062 Phosphoserine
    706-706 Phosphoserine
    712-712 Phosphotyrosine
    694-694 Phosphoserine
    680-680 Phosphoserine
Seqacetylation
    822-822 N6-acetyllysine
 
Prediction
NeProc
Disorder 1-930,957-960,984-993,1032-1086
Order 931-956,965-983,994-1031,1087-1163
ProS 1-70,299-304,311-324,471-476,579-583,641-646,715-729,767-781,865-870,881-889,902-924,1082-1086
AlphaFold
Disorder 1-1,21-33,38-45,65-313,315-315,323-712,729-901,960-963,1040-1040,1043-1080,1162-1163
Order 2-20,34-37,46-64,314-314,316-322,713-728,902-959,964-1039,1041-1042,1081-1161
SEG 129-148 ,168-185 ,188-197 ,376-393 ,424-468 ,568-577 ,627-640 ,645-663 ,694-708 ,836-894 ,1062-1082
Function
Function in SwissProt
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Biological Process
See also
Diagram with PDB data
tat/CCNT1/CDK9/AFF4/RNA(TAR)HIV-1 TAR loop in complex with Tat:AFF4:P-TEFb
tat/CCNT1/CDK9/AFF4/RNA(TAR)HIV-1 TAR loop in complex with Tat:AFF4:P-TEFb
tat/CCNT1/CDK9/AFF4crystal structure of P-TEFb complex with AFF4 and Tat