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IID50433
 UniprotP28327
ProteinRhodopsin kinase GRK1
GeneGRK1
OrganismBos taurus
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
561
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-25 Hetero dimer : P21457
 Evidence NMR 2i94 B Reference
       Region 2i94 B 1-16 order
       Region 2i94 B 1-2 high_rmsd
       Region 2i94 B 17-25 disorder
Seq 1-535 Homo tetramer :
 Evidence X-RAY 3qc9 A Reference
       Region 3qc9 A 1-30 disorder
       Region 3qc9 A 31-472 order
       Region 3qc9 A 473-489 disorder
       Region 3qc9 A 490-533 order
       Region 3qc9 A 534-535 disorder
 Evidence X-RAY 3qc9 B Reference
       Region 3qc9 B 1-30 disorder
       Region 3qc9 B 31-472 order
       Region 3qc9 B 473-494 disorder
       Region 3qc9 B 495-533 order
       Region 3qc9 B 534-535 disorder
 Evidence X-RAY 3qc9 C Reference
       Region 3qc9 C 1-30 disorder
       Region 3qc9 C 31-136 order
       Region 3qc9 C 137-141 disorder
       Region 3qc9 C 142-473 order
       Region 3qc9 C 474-489 disorder
       Region 3qc9 C 490-533 order
       Region 3qc9 C 534-535 disorder
 Evidence X-RAY 3qc9 D Reference
       Region 3qc9 D 1-30 disorder
       Region 3qc9 D 31-472 order
       Region 3qc9 D 473-489 disorder
       Region 3qc9 D 490-533 order
       Region 3qc9 D 534-535 disorder
Seq 1-535 Monomer :
 Evidence X-RAY 3c4z A Reference
       Region 3c4z A 1-30 disorder
       Region 3c4z A 31-137 order
       Region 3c4z A 138-140 disorder
       Region 3c4z A 141-480 order
       Region 3c4z A 481-484 disorder
       Region 3c4z A 485-488 order
       Region 3c4z A 489-491 disorder
       Region 3c4z A 492-533 order
       Region 3c4z A 534-535 disorder
 Evidence X-RAY 3t8o A Reference
       Region 3t8o A 1-29 disorder
       Region 3t8o A 30-475 order
       Region 3t8o A 476-480 disorder
       Region 3t8o A 481-487 order
       Region 3t8o A 488-492 disorder
       Region 3t8o A 493-532 order
       Region 3t8o A 533-535 disorder
 Evidence X-RAY 4wbo A Reference
       Region 4wbo A 1-27 disorder
       Region 4wbo A 28-481 order
       Region 4wbo A 482-490 disorder
       Region 4wbo A 491-533 order
       Region 4wbo A 534-535 disorder
 Evidence X-RAY 4wbo B Reference
       Region 4wbo B 1-29 disorder
       Region 4wbo B 30-482 order
       Region 4wbo B 483-491 disorder
       Region 4wbo B 492-533 order
       Region 4wbo B 534-535 disorder
 Evidence X-RAY 4wbo C Reference
       Region 4wbo C 1-30 disorder
       Region 4wbo C 31-473 order
       Region 4wbo C 474-493 disorder
       Region 4wbo C 494-531 order
       Region 4wbo C 532-535 disorder
 Evidence X-RAY 4wbo D Reference
       Region 4wbo D 1-29 disorder
       Region 4wbo D 30-136 order
       Region 4wbo D 137-141 disorder
       Region 4wbo D 142-483 order
       Region 4wbo D 484-489 disorder
       Region 4wbo D 490-531 order
       Region 4wbo D 532-535 disorder
Seq 1-561 Homo dimer :
 Evidence X-RAY 3c4w A The electron density of 3c4w-B chain indicated that Thr8 is phosphorylated. Reference
       Region 3c4w A 1-30 disorder
       Region 3c4w A 31-472 order
       Region 3c4w A 473-481 disorder
       Region 3c4w A 482-533 order
       Region 3c4w A 534-535 disorder
 Evidence X-RAY 3c4w B The electron density of 3c4w-B chain indicated that Thr8 is phosphorylated. Reference
       Region 3c4w B 1-4 disorder
       Region 3c4w B 5-137 order
       Region 3c4w B 138-139 disorder
       Region 3c4w B 140-480 order
       Region 3c4w B 481-488 disorder
       Region 3c4w B 489-533 order
       Region 3c4w B 534-535 disorder
 Evidence X-RAY 3c4x A Reference
       Region 3c4x A 1-29 disorder
       Region 3c4x A 30-472 order
       Region 3c4x A 473-480 disorder
       Region 3c4x A 481-533 order
       Region 3c4x A 534-535 disorder
 Evidence X-RAY 3c4x B Reference
       Region 3c4x B 1-30 disorder
       Region 3c4x B 31-134 order
       Region 3c4x B 135-142 disorder
       Region 3c4x B 143-475 order
       Region 3c4x B 476-495 disorder
       Region 3c4x B 496-533 order
       Region 3c4x B 534-535 disorder
 Evidence X-RAY 3c4y A Reference
       Region 3c4y A 1-29 disorder
       Region 3c4y A 30-137 order
       Region 3c4y A 138-141 disorder
       Region 3c4y A 142-393 order
       Region 3c4y A 394-395 disorder
       Region 3c4y A 396-474 order
       Region 3c4y A 475-493 disorder
       Region 3c4y A 494-532 order
       Region 3c4y A 533-535 disorder
 Evidence X-RAY 3c4y B Reference
       Region 3c4y B 1-29 disorder
       Region 3c4y B 30-115 order
       Region 3c4y B 116-117 disorder
       Region 3c4y B 118-135 order
       Region 3c4y B 136-141 disorder
       Region 3c4y B 142-192 order
       Region 3c4y B 193-200 disorder
       Region 3c4y B 201-472 order
       Region 3c4y B 473-493 disorder
       Region 3c4y B 494-532 order
       Region 3c4y B 533-535 disorder
 Evidence X-RAY 3c50 A Reference
       Region 3c50 A 1-29 disorder
       Region 3c50 A 30-483 order
       Region 3c50 A 484-488 disorder
       Region 3c50 A 489-533 order
       Region 3c50 A 534-535 disorder
 Evidence X-RAY 3c50 B Reference
       Region 3c50 B 1-29 disorder
       Region 3c50 B 30-478 order
       Region 3c50 B 479-486 disorder
       Region 3c50 B 487-533 order
       Region 3c50 B 534-535 disorder
 Evidence X-RAY 3c51 A Reference
       Region 3c51 A 1-30 disorder
       Region 3c51 A 31-137 order
       Region 3c51 A 138-140 disorder
       Region 3c51 A 141-474 order
       Region 3c51 A 475-487 disorder
       Region 3c51 A 488-533 order
       Region 3c51 A 534-535 disorder
 Evidence X-RAY 3c51 B Reference
       Region 3c51 B 1-30 disorder
       Region 3c51 B 31-113 order
       Region 3c51 B 114-116 disorder
       Region 3c51 B 117-137 order
       Region 3c51 B 138-141 disorder
       Region 3c51 B 142-268 order
       Region 3c51 B 269-270 disorder
       Region 3c51 B 271-277 order
       Region 3c51 B 278-286 disorder
       Region 3c51 B 287-394 order
       Region 3c51 B 395-396 disorder
       Region 3c51 B 397-405 order
       Region 3c51 B 406-412 disorder
       Region 3c51 B 413-475 order
       Region 3c51 B 476-490 disorder
       Region 3c51 B 491-533 order
       Region 3c51 B 534-535 disorder
 Evidence X-RAY 4l9i A Reference
       Region 4l9i A 30-533 order
 Evidence X-RAY 4l9i B Reference
       Region 4l9i B 30-30 disorder
       Region 4l9i B 31-478 order
       Region 4l9i B 479-488 disorder
       Region 4l9i B 489-533 order
 Evidence X-RAY 4pni A Reference
       Region 4pni A 1-29 disorder
       Region 4pni A 30-481 order
       Region 4pni A 482-493 disorder
       Region 4pni A 494-533 order
       Region 4pni A 534-561 disorder
SeqProS verified 1-16 Hetero dimer : P21457
       Region 2i94 B 1-16 order
       Region 3c4x A 1-29 disorder
SeqProS verified 5-29 The Thr(P) side chain forms hydrogen bonds with Gln73 and Glu93 of the RH domain and is sandwiched between the amino groups of Lys69 and Lys90. This structure is distinct from that of the GRK1 N-terminal peptide bound to Ca2+ and recoverin (PDB: 2i94), wherein residues 4-16 form an amphipathic helix. Homo dimer :
       Region 3c4w B 5-137 order
       Region 3c4x A 1-29 disorder
Seqphosphorylation
    5-5 Phosphoserine
    21-21 Phosphoserine; by PKA and autocatalysis
    8-8 Phosphothreonine
    488-488 Phosphoserine; by autocatalysis
    489-489 Phosphothreonine; by autocatalysis
 
Prediction
NeProc
Disorder 21-33,542-561
Order 1-20,34-541
ProS 21-26,542-545,555-561
AlphaFold
Disorder 1-4,20-29,544-561
Order 5-19,30-543
SEG 216-227
Function
Function in SwissProt
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:12686556, PubMed:16675451, PubMed:21299498). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
Biological Process
See also
Diagram with PDB data
RHO/SAGNMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state