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IID50441
 UniprotP39935
ProteinEukaryotic initiation factor 4F subunit p150
GeneTIF4631
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
952
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 35-49 Hetero dimer : P32588
 Evidence NMR 6z29 A Reference
       Region 6z29 A 35-49 order
       Region 6z29 A 35-37 high_rmsd
       Region 6z29 A 43-49 high_rmsd
Seqdisorder 1-199,226-249
 Evidence NMR Analysis of eIF4G1 1-249 secondary structure based on 13C chemical shifts, T1/T2 15N relaxation times and residual dipolar couplings (RDCs) revealed the presence of an alpha-helix within BOX3. eIF4G1 1-249 is predominantly disordered except for an alpha-helix in BOX3 (PMID: 36213119). Reference
       Region 1-199 disorder
       Region 226-249 disorder
Seq 391-488 Hetero dimer : IID50425Complex
 Evidence NMR 1rf8 B Reference
       Region 1rf8 B 391-488 order
       Region 1rf8 B 391-405 high_rmsd
       Region 1rf8 B 484-488 high_rmsd
Seqdisorder 391-488
 Evidence NMR The amide protons of the eIF4G1 393-490 fragment exhibited few, if any, structural constraints in the absence of eIF4E. (Note: the residues 393-490 in PMID:10409688 correspond to the residues 391-488 of P39935) Reference
       Region 391-488 disorder
Seq 572-854 Hetero tetramer : P10081
 Evidence X-RAY 2vso E Reference
       Region 2vso E 572-576 disorder
       Region 2vso E 577-582 order
       Region 2vso E 583-597 disorder
       Region 2vso E 598-685 order
       Region 2vso E 686-688 disorder
       Region 2vso E 689-716 order
       Region 2vso E 717-728 disorder
       Region 2vso E 729-802 order
       Region 2vso E 803-810 disorder
       Region 2vso E 811-853 order
       Region 2vso E 854-854 disorder
 Evidence X-RAY 2vso F Reference
       Region 2vso F 572-576 disorder
       Region 2vso F 577-582 order
       Region 2vso F 583-596 disorder
       Region 2vso F 597-685 order
       Region 2vso F 686-688 disorder
       Region 2vso F 689-716 order
       Region 2vso F 717-729 disorder
       Region 2vso F 730-802 order
       Region 2vso F 803-811 disorder
       Region 2vso F 812-852 order
       Region 2vso F 853-854 disorder
Seq 572-854 Hetero dimer : P10081
 Evidence X-RAY 2vsx E Reference
       Region 2vsx E 572-575 disorder
       Region 2vsx E 576-582 order
       Region 2vsx E 583-597 disorder
       Region 2vsx E 598-685 order
       Region 2vsx E 686-688 disorder
       Region 2vsx E 689-716 order
       Region 2vsx E 717-728 disorder
       Region 2vsx E 729-802 order
       Region 2vsx E 803-810 disorder
       Region 2vsx E 811-853 order
       Region 2vsx E 854-854 disorder
 Evidence X-RAY 2vsx F Reference
       Region 2vsx F 572-576 disorder
       Region 2vsx F 577-582 order
       Region 2vsx F 583-596 disorder
       Region 2vsx F 597-685 order
       Region 2vsx F 686-688 disorder
       Region 2vsx F 689-716 order
       Region 2vsx F 717-729 disorder
       Region 2vsx F 730-802 order
       Region 2vsx F 803-811 disorder
       Region 2vsx F 812-852 order
       Region 2vsx F 853-854 disorder
SeqProS verified 38-42 The residues with high_rmsd are excluded from the ProS. Hetero dimer : P32588
       Region 6z29 A 35-49 order
       Region 1-199 disorder
SeqProS verified 406-483 The residues with high_rmsd are excluded from the ProS. Hetero dimer : IID50425Complex
       Region 1rf8 B 391-488 order
       Region 391-488 disorder
Seqphosphorylation
    181-181 Phosphothreonine
    883-883 Phosphoserine
    888-888 Phosphothreonine
    908-908 Phosphoserine
    948-948 Phosphoserine
 
Prediction
NeProc
Disorder 1-198,227-448,454-461,471-602,714-729,862-952
Order 199-226,449-453,462-470,603-713,730-805,811-861
ProS 134-138,421-448,454-461,471-481,506-510,568-583,714-720,862-875,939-946
AlphaFold
Disorder 1-136,143-144,146-150,152-199,228-298,315-409,449-449,463-465,477-569,584-598,717-729,855-862,883-952
Order 137-142,145-145,151-151,200-227,299-314,410-448,450-462,466-476,570-583,599-716,730-854,863-882
SEG 22-33 ,35-46 ,64-84 ,98-110 ,173-191 ,214-226 ,279-292 ,302-324 ,374-388 ,396-410 ,522-532 ,557-568 ,780-794 ,864-876 ,879-899 ,923-938
Function
Function in SwissProt
Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4631 is probably essential when TIF4632 is missing.